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human foreskin fibroblast hffn  (System Biosciences Inc)

 
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    System Biosciences Inc human foreskin fibroblast hffn
    List of genes from expressed in the different stages of differentiation and maturation of SCs based on literature search.
    Human Foreskin Fibroblast Hffn, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast hffn/product/System Biosciences Inc
    Average 90 stars, based on 1 article reviews
    human foreskin fibroblast hffn - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "A Human Gonadal Cell Model From Induced Pluripotent Stem Cells"

    Article Title: A Human Gonadal Cell Model From Induced Pluripotent Stem Cells

    Journal: Frontiers in Genetics

    doi: 10.3389/fgene.2018.00498

    List of genes from expressed in the different stages of differentiation and maturation of SCs based on literature search.
    Figure Legend Snippet: List of genes from expressed in the different stages of differentiation and maturation of SCs based on literature search.

    Techniques Used: Wilms Tumor Assay, Binding Assay

    Graphic representation of Human Fibroblasts (HFs) cell reprogramming and iPSCs differentiation toward SLCs. AP, alkaline phosphatase; EBs, embrioid bodies; FGF9, fibroblast growth factor 9; IF, immunofluorescence; iPSCs, induced pluripotent stem cells; PGD2, prostaglandin 2; RNA-seq, next generation RNA sequencing; SLCs, Sertoli-like cells; SNL, SNL feeder cells.
    Figure Legend Snippet: Graphic representation of Human Fibroblasts (HFs) cell reprogramming and iPSCs differentiation toward SLCs. AP, alkaline phosphatase; EBs, embrioid bodies; FGF9, fibroblast growth factor 9; IF, immunofluorescence; iPSCs, induced pluripotent stem cells; PGD2, prostaglandin 2; RNA-seq, next generation RNA sequencing; SLCs, Sertoli-like cells; SNL, SNL feeder cells.

    Techniques Used: Immunofluorescence, RNA Sequencing

    (A) Immunofluorescence staining of iPSC colonies. Zoom-in details in right-down squares. Cells were stained with antibodies against OCT4, TRA-1-81, SSEA3, SSEA4, or SSEA1 and SOX2. Mounting medium contains DAPI stain. Pluripotency markers OCT4, TRA-1-81, and SOX2 positively marked the nuclei of cells. SSEA3 and SSEA4 were localized in the cytoplasmic area of colonies. SSEA1 expression was absent in colony nuclei but observed in the feeder cells. (B) RT-PCR analysis of pluripotential colonies for the genes DPPA2, DPPA4, ESG1, SOX2, and NANOG. Primers used for SOX2 and NANOG specifically detect transcripts from the endogenous genes, but not from the lentiviral transgenes. Four (1–4) different colonies are shown. HF, terminally differentiated fibroblasts; iPSC, induced pluripotent stem cells. (C,D) Teratoma formation analysis. Histology sections of teratoma stained with Hematoxylin & eosin. 1: mesoderm, 2: endoderm, 3: ectoderm. Scale bars 50 μm for (A,C) , and 100 μm for (D) .
    Figure Legend Snippet: (A) Immunofluorescence staining of iPSC colonies. Zoom-in details in right-down squares. Cells were stained with antibodies against OCT4, TRA-1-81, SSEA3, SSEA4, or SSEA1 and SOX2. Mounting medium contains DAPI stain. Pluripotency markers OCT4, TRA-1-81, and SOX2 positively marked the nuclei of cells. SSEA3 and SSEA4 were localized in the cytoplasmic area of colonies. SSEA1 expression was absent in colony nuclei but observed in the feeder cells. (B) RT-PCR analysis of pluripotential colonies for the genes DPPA2, DPPA4, ESG1, SOX2, and NANOG. Primers used for SOX2 and NANOG specifically detect transcripts from the endogenous genes, but not from the lentiviral transgenes. Four (1–4) different colonies are shown. HF, terminally differentiated fibroblasts; iPSC, induced pluripotent stem cells. (C,D) Teratoma formation analysis. Histology sections of teratoma stained with Hematoxylin & eosin. 1: mesoderm, 2: endoderm, 3: ectoderm. Scale bars 50 μm for (A,C) , and 100 μm for (D) .

    Techniques Used: Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction

    Immunofluorescence staining of SLC colonies with antibodies against SOX9. Mounting medium contains DAPI stain. SOX9 were expressed in SLC colonies. HF, terminally differentiated fibroblasts; HSerCs, human Sertoli cells; NT2, NT2d1 cells; SOX9, SRY (sex determining region Y)-box 9. Scale bars 50 μm.
    Figure Legend Snippet: Immunofluorescence staining of SLC colonies with antibodies against SOX9. Mounting medium contains DAPI stain. SOX9 were expressed in SLC colonies. HF, terminally differentiated fibroblasts; HSerCs, human Sertoli cells; NT2, NT2d1 cells; SOX9, SRY (sex determining region Y)-box 9. Scale bars 50 μm.

    Techniques Used: Immunofluorescence, Staining

    Secreted AMH quantification by ELISA. SLCs secreted AMH levels were significantly higher when compared with AMH secretion by HSerCs or NT2d1 cells. HF: terminally differentiated fibroblasts, HSerCs: primary human Sertoli cells, NT2d1: NT2d1 cells, SLCs: Sertoli like cells. n = 4 for SLCs and HSerCs, n = 2 for HFs and NT2d1. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001.
    Figure Legend Snippet: Secreted AMH quantification by ELISA. SLCs secreted AMH levels were significantly higher when compared with AMH secretion by HSerCs or NT2d1 cells. HF: terminally differentiated fibroblasts, HSerCs: primary human Sertoli cells, NT2d1: NT2d1 cells, SLCs: Sertoli like cells. n = 4 for SLCs and HSerCs, n = 2 for HFs and NT2d1. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001.

    Techniques Used: Enzyme-linked Immunosorbent Assay



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    human foreskin fibroblast hffn  (System Biosciences Inc)
    90
    System Biosciences Inc human foreskin fibroblast hffn
    List of genes from expressed in the different stages of differentiation and maturation of SCs based on literature search.
    Human Foreskin Fibroblast Hffn, supplied by System Biosciences Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human foreskin fibroblast hffn/product/System Biosciences Inc
    Average 90 stars, based on 1 article reviews
    human foreskin fibroblast hffn - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    List of genes from expressed in the different stages of differentiation and maturation of SCs based on literature search.

    Journal: Frontiers in Genetics

    Article Title: A Human Gonadal Cell Model From Induced Pluripotent Stem Cells

    doi: 10.3389/fgene.2018.00498

    Figure Lengend Snippet: List of genes from expressed in the different stages of differentiation and maturation of SCs based on literature search.

    Article Snippet: Human foreskin fibroblast (HFFn, PC501 A-HFF, System Biosciences Mountain View, CA, United States) were cultured in DMEM medium supplemented with 10% FBS and 1% Pen/Strep according to the manufacturer instructions.

    Techniques: Wilms Tumor Assay, Binding Assay

    Graphic representation of Human Fibroblasts (HFs) cell reprogramming and iPSCs differentiation toward SLCs. AP, alkaline phosphatase; EBs, embrioid bodies; FGF9, fibroblast growth factor 9; IF, immunofluorescence; iPSCs, induced pluripotent stem cells; PGD2, prostaglandin 2; RNA-seq, next generation RNA sequencing; SLCs, Sertoli-like cells; SNL, SNL feeder cells.

    Journal: Frontiers in Genetics

    Article Title: A Human Gonadal Cell Model From Induced Pluripotent Stem Cells

    doi: 10.3389/fgene.2018.00498

    Figure Lengend Snippet: Graphic representation of Human Fibroblasts (HFs) cell reprogramming and iPSCs differentiation toward SLCs. AP, alkaline phosphatase; EBs, embrioid bodies; FGF9, fibroblast growth factor 9; IF, immunofluorescence; iPSCs, induced pluripotent stem cells; PGD2, prostaglandin 2; RNA-seq, next generation RNA sequencing; SLCs, Sertoli-like cells; SNL, SNL feeder cells.

    Article Snippet: Human foreskin fibroblast (HFFn, PC501 A-HFF, System Biosciences Mountain View, CA, United States) were cultured in DMEM medium supplemented with 10% FBS and 1% Pen/Strep according to the manufacturer instructions.

    Techniques: Immunofluorescence, RNA Sequencing

    (A) Immunofluorescence staining of iPSC colonies. Zoom-in details in right-down squares. Cells were stained with antibodies against OCT4, TRA-1-81, SSEA3, SSEA4, or SSEA1 and SOX2. Mounting medium contains DAPI stain. Pluripotency markers OCT4, TRA-1-81, and SOX2 positively marked the nuclei of cells. SSEA3 and SSEA4 were localized in the cytoplasmic area of colonies. SSEA1 expression was absent in colony nuclei but observed in the feeder cells. (B) RT-PCR analysis of pluripotential colonies for the genes DPPA2, DPPA4, ESG1, SOX2, and NANOG. Primers used for SOX2 and NANOG specifically detect transcripts from the endogenous genes, but not from the lentiviral transgenes. Four (1–4) different colonies are shown. HF, terminally differentiated fibroblasts; iPSC, induced pluripotent stem cells. (C,D) Teratoma formation analysis. Histology sections of teratoma stained with Hematoxylin & eosin. 1: mesoderm, 2: endoderm, 3: ectoderm. Scale bars 50 μm for (A,C) , and 100 μm for (D) .

    Journal: Frontiers in Genetics

    Article Title: A Human Gonadal Cell Model From Induced Pluripotent Stem Cells

    doi: 10.3389/fgene.2018.00498

    Figure Lengend Snippet: (A) Immunofluorescence staining of iPSC colonies. Zoom-in details in right-down squares. Cells were stained with antibodies against OCT4, TRA-1-81, SSEA3, SSEA4, or SSEA1 and SOX2. Mounting medium contains DAPI stain. Pluripotency markers OCT4, TRA-1-81, and SOX2 positively marked the nuclei of cells. SSEA3 and SSEA4 were localized in the cytoplasmic area of colonies. SSEA1 expression was absent in colony nuclei but observed in the feeder cells. (B) RT-PCR analysis of pluripotential colonies for the genes DPPA2, DPPA4, ESG1, SOX2, and NANOG. Primers used for SOX2 and NANOG specifically detect transcripts from the endogenous genes, but not from the lentiviral transgenes. Four (1–4) different colonies are shown. HF, terminally differentiated fibroblasts; iPSC, induced pluripotent stem cells. (C,D) Teratoma formation analysis. Histology sections of teratoma stained with Hematoxylin & eosin. 1: mesoderm, 2: endoderm, 3: ectoderm. Scale bars 50 μm for (A,C) , and 100 μm for (D) .

    Article Snippet: Human foreskin fibroblast (HFFn, PC501 A-HFF, System Biosciences Mountain View, CA, United States) were cultured in DMEM medium supplemented with 10% FBS and 1% Pen/Strep according to the manufacturer instructions.

    Techniques: Immunofluorescence, Staining, Expressing, Reverse Transcription Polymerase Chain Reaction

    Immunofluorescence staining of SLC colonies with antibodies against SOX9. Mounting medium contains DAPI stain. SOX9 were expressed in SLC colonies. HF, terminally differentiated fibroblasts; HSerCs, human Sertoli cells; NT2, NT2d1 cells; SOX9, SRY (sex determining region Y)-box 9. Scale bars 50 μm.

    Journal: Frontiers in Genetics

    Article Title: A Human Gonadal Cell Model From Induced Pluripotent Stem Cells

    doi: 10.3389/fgene.2018.00498

    Figure Lengend Snippet: Immunofluorescence staining of SLC colonies with antibodies against SOX9. Mounting medium contains DAPI stain. SOX9 were expressed in SLC colonies. HF, terminally differentiated fibroblasts; HSerCs, human Sertoli cells; NT2, NT2d1 cells; SOX9, SRY (sex determining region Y)-box 9. Scale bars 50 μm.

    Article Snippet: Human foreskin fibroblast (HFFn, PC501 A-HFF, System Biosciences Mountain View, CA, United States) were cultured in DMEM medium supplemented with 10% FBS and 1% Pen/Strep according to the manufacturer instructions.

    Techniques: Immunofluorescence, Staining

    Secreted AMH quantification by ELISA. SLCs secreted AMH levels were significantly higher when compared with AMH secretion by HSerCs or NT2d1 cells. HF: terminally differentiated fibroblasts, HSerCs: primary human Sertoli cells, NT2d1: NT2d1 cells, SLCs: Sertoli like cells. n = 4 for SLCs and HSerCs, n = 2 for HFs and NT2d1. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001.

    Journal: Frontiers in Genetics

    Article Title: A Human Gonadal Cell Model From Induced Pluripotent Stem Cells

    doi: 10.3389/fgene.2018.00498

    Figure Lengend Snippet: Secreted AMH quantification by ELISA. SLCs secreted AMH levels were significantly higher when compared with AMH secretion by HSerCs or NT2d1 cells. HF: terminally differentiated fibroblasts, HSerCs: primary human Sertoli cells, NT2d1: NT2d1 cells, SLCs: Sertoli like cells. n = 4 for SLCs and HSerCs, n = 2 for HFs and NT2d1. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001.

    Article Snippet: Human foreskin fibroblast (HFFn, PC501 A-HFF, System Biosciences Mountain View, CA, United States) were cultured in DMEM medium supplemented with 10% FBS and 1% Pen/Strep according to the manufacturer instructions.

    Techniques: Enzyme-linked Immunosorbent Assay